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Reproducible from the start: How enzymes influence your data.
Tissue Dissociation is used to isolate cells from tissues. The aim is to get the highest number of cells to the best possible quality for further experiments. Critical issue is the quality of used enzymes, purified or crude.
To overcome the disadvantages of crude enzymes, we offer only highly purified enzyme systems and guarantee that – once tested – these work every time in the same way.
- PELOBiotech offers only highly purified Enzymes and enzyme systems.
- Results will be reproducible and further batch testing is redundant
- We are happy to stand by with our expertise
- We deliver fast and reliable
- We build bridges for research
Many of our high-quality products are even available in GMP grade PeloBiotech also says: “Custom Manufacturing is our daily business.”
Full expertise
We are the qualified, solution oriented experts you have been looking for. We develop for your cell culture needs.
Future oriented
Research means progress. We provide you with one of the best cell portfolio for your research needs.
Fearless bridge builders
With our products, we fill the gap and build a bridge between innovative research and the special requests of the clinical sector.
Our grade Enzyme products.
We are not only experts in the field of cells, we also really know a lot about enzymes and their benefits.
We have put together an overview of the most common areas of application for you:
Human Islet Applications, Rodent Islet Applications, Human Adipose Applications, Rodent Hepatocyte Applications and other Cells.
Together with our global Network of competent partners we strive for excellence. That is why we are proud to offer so many high quality, tested and highly purified enzyme products. We differentiate between three main product categories from which you can choose.
Collagenase & DE Collagenase
Here we are talking about highly purified enzymes.
The DE collagenase product family is characterized by increasing collagenase activities with the same amount of protease activity.
Protease
Here we are talking about highly purified enzymes.
Enzyme Blends
Here we are talking about mixtures of purified collagenase and purified proteases designed for special applications ...
... to increase reproducibility and lot-to-lot consistency. We offer tested and pretested blends for rodent islets, adipose derived stem cells and human hepatocytes.
What do you need to know about Collagenase and Protease.
Collagenase
Collagenases are enzymes that digest collagen the major fibrous component of animal and human extracellular connective tissue.
These enzymes are prepared from C. Histolyticum and are often used as crude enzyme preparation. The advantage of bacterial endopeptidases is that these enzymes will digest the insoluble collagen as well as soluble collagen fragments. The disadvantage of using crude collagenase preparations are the contaminating foreign enzyme activities (tryptic, caseinase and clostripain) which prevents reproducibility.
Avoiding this batch testing procedure repeatedly would be a big step forward and improve productivity. Highly purified enzyme preparations will make this lot procedure superfluous. That is why we are proud to be able to offer you highly purified enzyme preparations.
Protease
Thermolysin a neutral protease prepared from Bacillus thermoproteolyticus is a thermostable metalloproteinase used for isolation of islets from rodents, canine, monkey and human pancreas.
We are proud to offer you a wide variety of purified proteases. Among other high quality Thermolysin products, even animal-free Thermolysin in GMP grade.
This (animal component-free) is an aseptically dispensed, lyophilized purified protease from Bacillus thermoproteolyticus.
For islet research, isolation & transplantation.
Protease Enzyme Mixtures Successfully Used for Human Islet Isolation.
Together with our strong partners in the field of tissue dissociation, we offer lots of tools for your pancreas & islet research.
Purified bacterial collagenase and protease enzymes are commonly used to recover human islets from clinical grade pancreata for subsequent clinical islet transplantation. But the high cost of purified enzymes can often be prohibitive. In Summer 2019 VitaCyte, our partner for highly purified enzymes, successfully isolated islets from human research pancreata using enriched collagenase products supplemented with BP Protease (DE Collagenase 800 or Collagenase Gold-BP Protease mixtures) that cost significantly less than purified collagenase. Ask us to get your quality requirements and budget under one roof.
